In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy
نویسندگان
چکیده
منابع مشابه
In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy
We implemented in situ time-lapse Second Harmonic Generation (SHG) microscopy to monitor the three-dimensional (3D) self-assembly of collagen in solution. As a proof of concept, we tuned the kinetics of fibril formation by varying the pH and measured the subsequent exponential increase of fibril volume density in SHG images. We obtained significantly different time constants at pH = 6.5 ± 0.3 a...
متن کاملProgress on Three-dimensional Observation and in situ Observation Using Scanning Ion Microscopy
The progress on the focused ion beam fabrication method provides us several kinds of advanced techniques for the sample preparation of transmission electron microscopy. With improvement of the beam convergence and the increase of the ion accelerating voltage, the ability of its scanning ion microscope becomes glade up with a good image resolution. The scanning ion microscope is now powerful too...
متن کاملCollagen fibrillogenesis in the presence of lanthanides.
Following removal of most of the telopeptide regions with pepsin, bovine dermal collagen gelled more slowly to form fibrils with a weak banding pattern. The reduction in gelling rate reflected an increase in the length of the nucleation phase and a lower rate of turbidity increase during the growth phase; the activation energy of both phases was increased. Lanthanide ions, phosphate, or, to a l...
متن کاملFibrillogenesis in continuously spun synthetic collagen fiber.
The universal structural role of collagen fiber networks has motivated the development of collagen gels, films, coatings, injectables, and other formulations. However, reported synthetic collagen fiber fabrication schemes have either culminated in short, discontinuous fiber segments at unsuitably low production rates, or have incompletely replicated the internal fibrillar structure that dictate...
متن کاملMonitoring Fibrous Scaffold Guidance of Three-Dimensional Collagen Organisation Using Minimally-Invasive Second Harmonic Generation
The biological and mechanical function of connective tissues is largely determined by controlled cellular alignment and therefore it seems appropriate that tissue-engineered constructs should be architecturally similar to the in vivo tissue targeted for repair or replacement. Collagen organisation dictates the tensile properties of most tissues and so monitoring the deposition of cell-secreted ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2012
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.3.001446